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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
1000.00 | 2.195 | 2.110 |
500.00 | 1.657 | 1.572 |
250.00 | 1.136 | 1.051 |
125.00 | 0.828 | 0.743 |
62.50 | 0.496 | 0.411 |
31.25 | 0.315 | 0.230 |
15.63 | 0.189 | 0.104 |
0.00 | 0.085 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 80-95% | 87% |
EDTA plasma(n=5) | 88-103% | 95% |
Heparin plasma(n=5) | 95-107% | 101% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 88-97% | 91-105% | 87-98% | 95-105% |
EDTA plasma(n=5) | 84-99% | 83-101% | 86-99% | 93-101% |
Heparin plasma(n=5) | 89-105% | 87-98% | 92-100% | 88-104% |
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